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Preparation and application of nanoparticles and nanofibres with natural UV filters
Plachá, Monika ; Skoumalová, Petra (referee) ; Márová, Ivana (advisor)
The presented diploma thesis is focused on preparation of nanoparticles and nanofibres with natural UV filters. Liposomes with encapsulated aqueous, ethanol and lipid extracts were prepared. Nanofibers from PHB containing lipid extract were prepared too. As a part of this work, an overview of natural sources with potential effects as UV filters were introduced. Moreover, nanoparticles and nanofibers and methods of their characterization were described. Size, polydisperse index and colloid stability of prepared nanoparticles were characterized via DLS. In experimental part aqueous, ethanol and lipid extracts were prepared from roasted coffee, green coffee and cascara. These extracts were spectrophotometrically characterized for the content of polyphenols, flavonoids, antioxidant activity, tannins and their SPF. Liposomes and liposomes containing PHB with these extracts were prepared and the encapsulation effectivity, short–term and long–term stability as well as SPF of nanoparticles were determined. Nanofibers from PHB containing lipid extracts were prepared via electrospinning and forcespinning methods. Prepared nanofibers were examined via FTIR–ATR. Antioxidant activity, short–term and long–term stability were determined spectrophotometrically. From selected nanoparticles, emulsions and gels were prepared and their SPF was also determined. Three types of emulsions with the best SPF were selected and tested on volunteers. Sedimentation stability of emulsions was tested by analytical centrifuge. Finally, cytotoxicity of selected nanoparticles and nanofibers was tested via MTT assay using human keratinocytes.
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Use of some microbial oils in foods and cosmetics
Požgayová, Viktória ; Szotkowski, Martin (referee) ; Márová, Ivana (advisor)
This bachelor thesis focuses on preparation of microbial oils from oleaginous yeasts and the subsequent characterization of their properties and biological effects on organisms. The thesis is divided into two main parts, theoretical and experimental part. The theoretical part provides an overview of information about microbial oils and the possibilities of their current or future applications in food and cosmetic industry. It also describes other lipophilic metabolites synthesized by carotenogenic yeasts and briefly characterizes the carotenogenic yeast strains as well as analytical methods used in the experimental part. Experimental part was focused on the process of optimization of the extraction of lipophilic active compounds from yeast biomass. The best option proved to be the combination of three solvents such as dimethyl sulfoxide, hexane, and ethanol. Using this approach, the extracts were prepared from Rhodotorula kratochvilovae, Rhodotorula mucilaginosa, Rhodosporidium toruloides, Cystofilobasidium macerans and Sporobolomyces pararoseus biomass. All of the prepared extracts were characterised based on the content of lipophilic metabolites by UV-VIS spectrophotometry and by analysis on HPLC/PDA and GC/FID. Furthermore, the antioxidant activity, SPF, antimicrobial effect, and short-term stability of these extracts were determined. The best temperature to preserve these extracts proved to be 7 °C. Three extracts with the highest SPF values were chosen and added to the prepared cosmetic emulsion which was characterized and the sensory analysis was carried out. The MTT cytotoxicity assay on human HaCaT keratinocytes was realized for the three extracts that were added to the prepared cosmetic product as well as for their combination.
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Preparation and characterization of biomaterials with natural UV filtres
Pavelková, Renata ; Čarnecká, Martina (referee) ; Márová, Ivana (advisor)
This presented diploma thesis is focuses on preparation and characterization of biomaterials with natural UV filters. The theoretical part is aimed at effects of sunlight on the skin and how to avoid these effects by using cosmetic products. The materials and methods for extraction of lipid part of the selected vegetable and microorganism species and methods for preparation and characterization of nanoparticles and emulsions were characterized. The practical part deals with extraction of lipid part of selected vegetable and microorganism species. The liposome particles with encapsulated active compounds were prepared. The particle size, polydispersity index, colloidal stability, encapsulation efficiency, SPF, antioxidant capacity, phospholipid content and cytotoxic effect on human keratinocytes were observed. These particles were used for preparation of the resulting emulsions. Their protection level on UV light were set and stability were tested by using analytical centrifugation.
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Development and optimization of stable recipe of tanning lotion.
Němečková, Lenka ; Pávek, Stanislav (referee) ; Pekař, Miloslav (advisor)
This bachelor´s thesis is focused on problems of development and optimalization of stable formula of sunscreen with a sun protective factor SPF 15, which is produced in Altermed Olomouc company. In theoretical part are defined terms as cosmetics, laws, which deal with cosmetics and sunscreens especially. Somatic types of the skin, their reaction to UV rays and their protection against them are there described as well. In experimental part there summarized all requirements, which are necessary to realize for introduction of preparation into the market place and during long term use it must continue to pass all laws, health, marketing and functional claims. I produced a sample of stable sunscreen with protective factor SPF 15 in practical part. This sample was tested in accredited laboratory for all aspects which are necessary for legislation. The results of these testing are noticed in laboratory protocol.
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Analysis and Visualization of e-Mail Marketing Campaigns
Urbanczyk, Martin ; Holkovič, Martin (referee) ; Hynek, Jiří (advisor)
The goal of this thesis is evaluating Posílátko.cz application and making extensions on the basis of thorough analysis and comparison of other similar products. This extensions are going to lead into increased user awareness of campagins state, so user can react accordingly. Extensions are going to be founded mostly on statistics data. Next I am going to implement campaign summary documents, where people can find all the data without logging into the application. So user can take this documents and present it easily on corporate consultations.
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Preparation of microbial, plant and algal extracts to use in cosmetics for infants
Janderová, Šárka ; Dzurická, Lucia (referee) ; Márová, Ivana (advisor)
This bachelor thesis deals with the preparation of a cosmetic product for infants with the addition of active substances from cyanobacteria, microalgae, or plants. In the theoretical part, a review was made, which not only summarizes the basic information about these organisms, but also the antioxidants contained in these microorganisms. In the practical part, several types of extracts from cyanobacteria, algae and plants were prepared and characterized. The content of phenolic substances, chlorophylls and content of carotenoids were measured spectrophotometrically. Content of soluble proteins was determined by Hartree-Lowry method and the fatty acid profile in biomass was obtained by GC. Furthermore, the SPF factor was measured using the spectrophotometric method the Mansur equation. Antioxidant activity was determined by using the TEAC method. Finally, two of the best extracts were selected for the preparation of cosmetic emulsions. The sensory analysis of prepared emulsions was performed for the period of two weeks.
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Biological effects of complex extracts from yeast and algae biomass
Bočán, David ; Byrtusová, Dana (referee) ; Skoumalová, Petra (advisor)
This bachelor thesis was focused on optimalization of extractions of active compounds from microalgae and carotenogenic yeasts. Other part of this work was focused on characterization of these extracts and their tests of cytotoxicity on human keratinocytes. In theoretical part a literary research which summarizes the basic properties and cultivation conditions of used microorganisms was conducted. Then there were listed an examples of antioxidant compounds found in these microorganisms as well as their properties. Finally the term cytotoxicity and methods of its measurement were clarified. The practical part of this thesis consists of series of extractions from mentioned microorganisms. These extracts were characterized by the content determination of phenolic and flavonoid compounds and also chlorophyll and carotenoid compounds. Determination of antioxidant capacity and SPF were done too. Lastly the cytotoxicity of chosen extracts was measured using the MTT test.
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Isolation and characterizaation of oil from some types of coffee
Vašků, Barbora ; Hoová, Julie (referee) ; Skoumalová, Petra (advisor)
Coffee, extract, oil, phenolic compounds, flavonoids, antioxidant capacity, SPF, critical wavelenght.
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Encapsulation of plant extracts containing phenolic compounds into nanoparticles and nanofibers
Petrželková, Markéta ; Bokrová, Jitka (referee) ; Skoumalová, Petra (advisor)
The presented bachelor thesis was focused on the encapsulation of extracts containing phenolic substances in order to apply these materials in cosmetics. At work were prepared aqueous, ethanol and lipid extracts of coffee and cocoa. All extracts were characterized on the content of all polyphenols and flavonoids. Their antioxidant aktivity and SPF were also determined. Then the selected extracts were encapsulated into liposomes and polyhydroxybutyrate nanofibres. The basic characteristics of the prepared liposomes were also observed and also their SPF and antioxidant aktivity were monitored. The highest antioxidant aktivity and SPF had liposome particles containing ethanol cocoa extracts. The liposome particles were applied to cosmetic emulsion. These creams evince high antioxidant acitivity and stability determined by analytical centrifugation. Also the prepared nanofibres had high antioxidant aktivity and confirmed the gradual release of the active substances. In conclusion the test cytotoxicity on human keratinocytes confirmed the safety of prepared nanomaterials using the MTT test, which are therefore suitable for cosmetic applications.
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Preparation of microbial, plant and algal extracts to use in cosmetics for infants
Janderová, Šárka ; Dzurická, Lucia (referee) ; Márová, Ivana (advisor)
This bachelor thesis deals with the preparation of a cosmetic product for infants with the addition of active substances from cyanobacteria, microalgae, or plants. In the theoretical part, a review was made, which not only summarizes the basic information about these organisms, but also the antioxidants contained in these microorganisms. In the practical part, several types of extracts from cyanobacteria, algae and plants were prepared and characterized. The content of phenolic substances, chlorophylls and content of carotenoids were measured spectrophotometrically. Content of soluble proteins was determined by Hartree-Lowry method and the fatty acid profile in biomass was obtained by GC. Furthermore, the SPF factor was measured using the spectrophotometric method the Mansur equation. Antioxidant activity was determined by using the TEAC method. Finally, two of the best extracts were selected for the preparation of cosmetic emulsions. The sensory analysis of prepared emulsions was performed for the period of two weeks.
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